吳映明; 陳愛葵; 彭錦紅; 梁美玲
廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系 廣州510303; 廣州510303; 廣州510303; 廣州510303

【中文摘要】 利用苯酚-硫酸法對破壁處理后的靈芝孢子粉多糖含量進行測定,結(jié)果表明采用超低溫凍融處理和超聲波處理進行靈芝孢子粉破壁均具有效果好、成本低及操作簡便等特點;增加凍結(jié)-解凍循環(huán)次數(shù)在一定程度上能提高多糖含量,循環(huán)次數(shù)以5次～7次為宜;將超聲波處理法和超低溫凍融法很好地結(jié)合起來,其多糖釋放更高,效果更佳,此方法更具實用價值。

【英文摘要】 The measurement of polysaccharides in sporoderm-broken spore was analysed by the method of phenol-sulfuric acid. The result shows that it is an effective, economic and facile way to break the cellular wall with thawing and freezing of ultra-low temperture or ultrasonic. And the circulating times of thawing and freezing can increase the contents of polysaccharides in a certain degree and the sug-gested number is 5～7. The method of combining ultrasonic with thawing and freezing of ultra-low temperture commendably can release the polysaccharides of its contents higher and be of more practical value.

【中文關(guān)鍵詞】 靈芝孢子粉; 靈芝多糖; 超低溫凍融; 超聲波
【英文關(guān)鍵詞】 Ganoderma lucidum spore powder; Polysaccharides in Ganoderma lucidum; Thawing and freezing of ultra-low temperture; Ultrasonic

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:ZSYJ.0.2008-03-016