黃龍花; 楊小兵; 張智; 胡惠萍; 張一帆; 吳清平
廣東粵微食用菌技術有限公司; 廣東省微生物研究所; 廣東省菌種保藏與應用重點實驗室

【中文摘要】 針對11個本所保藏靈芝屬菌株及9個靈芝屬野生菌株的ITS序列,應用特異引物進行PCR擴增,對其產(chǎn)物進行測序,并從GenBank上下載2條赤芝(Ganoderma luncidum)、1條黑紫靈芝(Ganoderma japonicum)和1條無柄紫靈芝(Ganoderma mastoporum)的ITS序列,以猴頭菇(Hericium erinaceum)為外類群,用NJ(Neighbor-Joining)法構建系統(tǒng)發(fā)育樹,結果表明,20個靈芝屬菌株聚為4個簇,其中赤芝組和樹舌亞屬的菌株各自聚為1組,其組內(nèi)同源性均達99.7%以上,紫靈芝組的菌株分成2組:無柄紫靈芝和黑紫靈芝。系統(tǒng)發(fā)育分析也表明僅根據(jù)形態(tài)學特征難以將靈芝屬菌株進行有效的分類,利用分子生物學的技術手段對靈芝菌株進行分類是一種更有效的方法,ITS序列分析在近緣種的分類鑒定上很有價值。

【英文摘要】 ITS sequence analysis of 11 preserved strains and 9 wild strains of Ganoderma spp.were investigated in this study. ITS sequences were produced from 20 strains of Ganoderma spp.by cloning sequencing of PCR products.Data were combined with ITS sequences from 6 related strains,including Ganoderma luncidum,Ganodermajaponicum,Ganoderma mastoporum and Hericium erinaceum,obtained from GenBank to construct a phylogenetic tree using the Neighbor-joining method with Hericium erinaceum as the outgroups.Four distinct groups were identified including the sect.Ganoderma,the sect.subgenus Elfvingia, the sect.Phaeonema.Similarity value among sects Ganoderma and subgenus Elfvingia strains were>99.7%.Analysis of molecular data is a more effective and useful approach for studying the taxonomy of Ganoderma,and for establishing phylogenefic relationships within the genus,compared to methods based on morphology of fruiting body.

【中文關鍵詞】 靈芝屬; ITS序列; 系統(tǒng)發(fā)育樹; 鑒定
【英文關鍵詞】 Ganoderma; ITS sequences; Phylogenetic tree; Identification
【基金】廣州市科技支撐計劃項目(2008Z1-E011);; 國際科技合作項目(2009DFA30830)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年01期 【DOI】CNKI:SUN:ZSYJ.0.2010-01-021