陳明杰 ; 譚琦 ; 汪昭月 ; 馮志勇 ; 賀冬梅 ; 嚴(yán)培蘭 ; 關(guān)斯民 ; 潘迎捷
上海市農(nóng)業(yè)科學(xué)院食用菌研究所 ; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開放實(shí)驗(yàn)室 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室 上海 201106

【中文摘要】 本文應(yīng)用RAPD技術(shù)與聚類分析方法,對(duì)香菇細(xì)胞融合菌株F1及親本S1、465進(jìn)行遺傳分析.根據(jù)DNA擴(kuò)增的指紋圖譜,估算融合子與親本的DNA相似系數(shù)并構(gòu)建遺傳相關(guān)性聚類圖.結(jié)果表明,RAPD技術(shù)與聚類分析方法可應(yīng)用于香菇細(xì)胞融合菌株的遺傳鑒定.

【英文摘要】 Fusant Fl of Lentinula edodes and parent strains S1 and 465 were analyzed by RAPD technique and cluster analysis. According to DNA fingerprint pattern generated from DNA amplification, DNA similarity coefficient of fusant and parent strains was calculated and the dendrogram of genetic relationship was constructed. The results suggested that RAPD technique and cluster analysis could be used for genetic identification of L. edodes fusants.

【中文關(guān)鍵詞】 香菇; DNA相似性; 遺傳相關(guān)性; 細(xì)胞融合
【英文關(guān)鍵詞】 Lentinula edodes; DNA similarity; Genetic relationship; Fusion

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-003