張慧麗; 楊松; 李玉; 王彥敏; 王力; 劉宏生
遼寧大學(xué)生命科學(xué)系; 遼寧沈陽(yáng)軍區(qū)藥品檢驗(yàn)所; 遼寧大學(xué)生命科學(xué)系; 遼寧大學(xué)生命科學(xué)系; 遼寧大連野生靈芝生物科技有限公司; 遼寧大學(xué)生命科學(xué)系 沈陽(yáng)110036; 沈陽(yáng)110026; 沈陽(yáng)110036; 沈陽(yáng)110036; 大連116038; 沈陽(yáng)110036

【中文摘要】 探討用超聲提取法提取樺褐孔菌中樺褐孔菌多糖的工藝條件及樺褐孔菌多糖對(duì)肝癌SMMC7721細(xì)胞株的抗增殖作用。設(shè)計(jì)正交實(shí)驗(yàn),對(duì)樺褐孔菌的超聲提取工藝進(jìn)行研究,并用MTT比色法研究樺褐孔菌多糖對(duì)肝癌SMMC7721細(xì)胞株的抗增殖作用。樺褐孔菌多糖超聲提取的最佳工藝條件為95℃、15倍水、20kHz、超聲60min,樺褐孔菌多糖在1.0￣16.0μg/mL范圍內(nèi)對(duì)肝癌SMMC7721細(xì)胞株的增殖均有抑制作用,且表現(xiàn)出濃度相關(guān)性。

【英文摘要】 OBJECTIVE To investigate the optimal purified protocol of extracting Inonotus obliquus polysaccharide using ultrasonication and the effect of the extractions from Inonotus obliquus polysaccharide on anti-proliferation for SMMC7721 cells of liver cancer.METHORD The method of orthogonal analysis was used to extract Inonotus obliquus polysaccharid with ultrasonication,The methods of MTT was used to study the anti-proliferative effect of the polysaccharide on the hepatocarcinoma SMMC7721cells.RESULT The best condition used in the course of extraction of Inonotus obliquus polysaccharide was: the temperature was 95℃,the volume of water was 15 times,the frequency of ultrasonication on the extraction was 20kHz,the optimal duration of extraction was 60min.The Inonotus obliquus polysaccharide had the inhibiting action on SMMC7721 cells of liver cancer line in the concentration of 1.016.0ug/mL by means of MTT assay,and exhibited a strong dependence on concentration.

【中文關(guān)鍵詞】 樺褐孔菌多糖; 超聲提取; 肝癌細(xì)胞抑制
【英文關(guān)鍵詞】 Inonotus obliquus polysaccharide; ultrasonication; anti-proliferation of hepatocarcinoma

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2006年02期 【DOI】CNKI:SUN:ZSYJ.0.2006-02-011