毛寧; 陳榮; 王澤生
福建師大生物工程學院; 福建師大激光研究所; 福建省輕工研究所 福州 350007

【中文摘要】 用He-Ne激光和半導體激光輻照四株雙孢蘑菇的菌絲體,以不處理為對照。取輻照部位轉接PDA培養(yǎng)基培養(yǎng)12～15天,用聚丙烯酰胺凝膠電泳(PAGE)分析菌絲體酯酶同工酶譜。結果表明:He-Ne激光處理不改變蘑菇菌絲體酯酶同工酶主要區(qū)帶(F與S區(qū))的遷移率(Rf)。但是,20mwHe-Ne激光10分鐘劑量不僅使酯酶同工酶活力明顯增強,而且使得M區(qū)酶帶數目增加。半導體激光對雙孢蘑菇的輻照效果不及He-Ne激光,且不同功率之間效果差異不顯著。

【英文摘要】 Using different power, different dosage' s He-Ne laser (632.8nm) and semiconductor laser (805nm)to irradiate mycelium of four Agaricus bisporus strains. And using unirradiate strain as contrast. Taking out some mycelium from irradiation position and then transfer them into PDA culture medium, cul- turing 1215 days. We use the polyacrylamide slab gel electrophoresis (PAGE) to examine the esterase (EST) isozymes of mycelium. We can see from the result that laser irradiating cannot change the Rf of main bands (F＆S zones) of mycelium EST. However, 20mw He-Ne, 10 minutes irradiation can not only raise the activity of EST distinctly, but also increase the number of enzyme zone of M zone. The difference be- tween the semiconductor irradiation of 140mw、10 minutes and 20mw、10 minutes is not remarkable. And the irradiating effect of He-Ne laser is better than that of semiconductor laser.

【中文關鍵詞】 He—Ne激光; 半導體激光; 雙孢蘑菇酯酶; 同工酶
【英文關鍵詞】 He-Ne laser; Semiconductor laser; Agaricus bisporus; EST
【基金】國家自然科學基金;; 福建省自然科學基金

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1997年02期 【DOI】CNKI:SUN:ZSYJ.0.1997-02-006