一種不經(jīng)熱浴從雙孢蘑菇子實(shí)體中提取DNA的新方法(英文)
A New Protocol for Extracting DNA from Agaricus bisporus Fruit Bodies without Thermal Incubation

【作者】 姬宏超； 萬艷娟； 莫美華； 徐學(xué)鋒；

【Author】 JI Hongchao,WAN Yanjuan,MO Meihua,XU XuefengCollege of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642,China

【機(jī)構(gòu)】 華南農(nóng)業(yè)大學(xué)食品學(xué)院；

【摘要】 介紹一種不經(jīng)熱浴快速從雙孢蘑菇子實(shí)體中提取DNA的新方法,并與傳統(tǒng)的CTAB和SDS法相比較,結(jié)果顯示:該方法得到的DNA完整性好,時(shí)間短,步驟少,有毒試劑用量少,在提取DNA的質(zhì)量、得率、時(shí)間及完整性等方面都優(yōu)于傳統(tǒng)的CTAB和SDS法,為食藥用菌子實(shí)體DNA的提取參考。

【Abstract】 A new,simple and rapid method for obtaining DNA from Agaricus bisporus fruit bodies is described and compared with the widely used CTAB and SDS methods in terms of yield,DNA quality and integrity,and duration of extraction time.Total DNA quality was estimated by restriction endonuclease digestion and by ITS,ISSR and RAPD analyses using standard protocols.Our data demonstrated that the new method renders improved yields of total DNA of high quality and integrity,and is less time-consuming than ex...

【關(guān)鍵詞】 雙孢蘑菇； DNA提??； 內(nèi)切酶消解；
【Key words】 Agaricus bisporus； DNA isolation； restriction endonuclease digestion；

【基金】 Supported by National Natural Science Foundation of China(No.30800774)

【分類號】S646