【作者】 潘鴻輝　謝意珍　李向敏　張智　余雄濤　焦春偉　蔡勉華　楊小兵

【Author】 PAN Hong-hui,XIE Yi-zhen,LI Xiang-min,ZHANG Zhi,YU Xiong-tao,JIAO Chun-wei,CAI Mian-hua,YANG Xiao-bing(Guangdong Institute of Microbiology,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Yuewei Edible Fungi Technology Co.Ltd.,Guangzhou Guangdong 510070)

【機構】 廣東省微生物研究所廣東省菌種保藏與應用重點實驗室廣東省微生物應用新技術公共實驗室;廣東粵微食用菌技術有限公司

【摘要】 應用3種腫瘤細胞模型對未破壁靈芝孢子、機械破壁靈芝孢子和全靈芝剝壁孢子進行抗腫瘤試驗研究。結果表明,未破壁及不同破壁方式的靈芝孢子對人惡性乳腺癌細胞(MT-1)、人惡性淋巴癌細胞株(Jurkat)、人惡性腦腫瘤細胞(U343)均有不同程度的抑制作用,抑制效果強弱為全靈芝剝壁孢子>機械破壁靈芝孢子>未破壁靈芝孢子,表明破壁的確能提高靈芝孢子對腫瘤細胞的抑制作用,而且選擇破壁的方法也很重要,生物酶法破壁能使靈芝孢子的生物活性成分得到充分釋放,所以大大提高靈芝孢子的功效。

【Abstract】 The objective of this paper was to compare the inhibitory effects in intact Ganoderma spores with sporoderm-broken spores which were broken by enzymatic method and physical method on three kind of tumour cells including MT-1(Human Breast Cancer),Jurkat(Human T cell lymphoblast-like cell line) and U343(Human astrocytoma cell line).The experiments indicated that the inhibitory effects of Ganoderma spores on cancer cell growth were sporoderm-broken spores(broken by en-zymatic method) > sporoderm...

【關鍵詞】 靈芝孢子;破壁;腫瘤細胞

【Key words】 Ganoderma spores;Sporoderm-broken;Tumour cells

【基金】 廣東省科技型中小企業(yè)技術創(chuàng)新基金項目(ZX032280);;廣東省中國科學院全面戰(zhàn)略合作項目(2009B091300139)

【文獻出處】 中國食用菌, Edible Fungi of China, 編輯部郵箱 , 2010年05期

【DOI】CNKI:SUN:ZSYJ.0.2010-05-017