【作者】周帥; 唐慶九; 楊焱; 賈薇; 劉艷芳; 唐傳紅; 馮娜; 劉方; 張勁松

【Author】ZHOU Shuai; TANG Qingjiu; YANG Yan; JIA Wei; LIU Yanfang; TANG Chuanhong; FENG Na; LIU Fang; ZHANG Jinsong National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Research Center of Natural Medicinal Resources; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China

【作者機構】國家食用菌工程技術研究中心; 農業(yè)部應用真菌資源與利用重點開放實驗室; 上海市農業(yè)遺傳育種重點開放實驗室; 上海市農業(yè)科學院天然藥用資源研究與開發(fā)中心; 上海市農業(yè)科學院食用菌研究所

【摘要】 以藥用真菌姬松茸(Agaricus blazei)、雞腿菇(Coprinus comatus)、猴頭(Hericium erinaceus)、灰樹花(Grifola frondosa)和靈芝(Ganoderma lucidum)的水提多糖為材料,采用二喹林甲酸法、考馬斯亮藍法、福林-酚法、紫外分光光度法、離子色譜法和傳統(tǒng)凱氏定氮法對其中的蛋白含量進行測定和比較分析。結果表明,不同測定方法獲得的蛋白含量測定值有差異;筆者推薦使用離子色譜法進行精確蛋白含量測定,采用福林-酚法進行快速趨勢比較測定。

【Abstract】 Protein in crude polysaccharide material from five medicinal fungi (Agaricus blazei,Coprinus comatus,Hericium erinaceus,Grifola frondosa,Ganoderma lucidum),prepared by hot (100 ℃) aqueous extraction,was determined using six procedures:bicinchoninic acid. assay,Bradford,Lowry,Kjeldahl,UV spectrophotometry and high performance anion exchange chromatography (HAPEC-PAD). Different protein values were obtained using each procedure,and it is proposed that HAPEC-PAD and the Lowry method be adopted for the accurate and approximate determination of protein in fungal polysaccharide preparations,respectively.

【關鍵詞】 藥用真菌; 多糖; 蛋白含量; 福林-酚法; 離子色譜法

【Key words】 Medicinal fungi; polysaccharide; protein content; Lowry method; high performance anion exchange chromatography

【基金】上海市農業(yè)科學院青年基金項目的部分研究內容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-017

【分類號】S567.3