李翠翠; 郭立忠; 盧偉東; 董偉
青島農(nóng)業(yè)大學(xué)山東省應(yīng)用真菌省級(jí)重點(diǎn)實(shí)驗(yàn)室
【中文摘要】 利用RAPD及SRAP分子標(biāo)記技術(shù)對(duì)從國(guó)內(nèi)外收集到的12個(gè)真姬菇菌株進(jìn)行遺傳多樣性分析。結(jié)果表明,20條RAPD引物共擴(kuò)增出多態(tài)性條帶285條,18對(duì)SRAP引物共擴(kuò)增出多態(tài)性條帶176條。在相似系數(shù)0.800水平時(shí),RAPD和SRAP分子標(biāo)記分別將12個(gè)真姬菇菌株分為5個(gè)和6個(gè)類(lèi)群。兩種方法均適用于真姬菇種內(nèi)鑒定,而SRAP標(biāo)記較RAPD標(biāo)記穩(wěn)定性好,更適于真姬菇的種內(nèi)鑒定。
【英文摘要】 Randomly amplified polymorphic DNA(RAPD)and sequence-related amplified polymorphisms(SRAP)were used to identify 12 strains of Hypsizygus marmoreus Sing.A total of 285 and 176 polymorphic bands were amplified by 20 RAPD primers and 18 SRAP primer pairs,respectively.Two dendrograms based on UPGMA(unweighted pair group method with arithmetic average)cluster analysis using the amplification data revealed that,at the similarity level of 0.8,the 12 strains clustered into five and six groups,respectively.We conclude that both methods are suitable for discriminating among strains of H.marmoreus,and that the SRAP markers were more effective in reflecting genetic diversity.
【中文關(guān)鍵詞】 真姬菇; RAPD; SRAP; 聚類(lèi)分析
【英文關(guān)鍵詞】 Hypsizygus marmoreus; RAPD; SRAP; clustering analysis
【基金】國(guó)家科技支撐計(jì)劃子課題“秸稈栽培食用菌高產(chǎn)高效技術(shù)模式與示范”(編號(hào):2007BAD89B09-15);; 公益性行業(yè)科研專(zhuān)項(xiàng)子課題“非熱滅菌秸稈食用菌培養(yǎng)基關(guān)鍵技術(shù)研究”(編號(hào):200803033-B0604)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-005