劉西周; 郭成金
天津師范大學(xué)化學(xué)與生命科學(xué)學(xué)院

【中文摘要】 采用正交設(shè)計(jì)方法以血耳Tremella sanguinea菌絲體的生物量為測(cè)量指標(biāo),對(duì)血耳菌絲體液體培養(yǎng)基進(jìn)行了篩選,結(jié)果表明血耳菌絲體液體培養(yǎng)的最適培養(yǎng)基為蔗糖7.5g·L-1、麥芽糖7.5g·L-1、麥麩7.5g·L-1、牛肉膏3.5g·L-1、MgSO4·7H2O0.5g·L-1、KH2PO41.5g·L-1、VBl4mg·L-1,pH自然。28℃培養(yǎng)96h,其生物量可達(dá)4.29g·L-1。

【英文摘要】 Taking biomass of mycelium in liquid medium of Tremella sanguinea as measurement index, the optimum liquid medium for mycelium of Tremella sanguinea by L9 (34) orthogonal design was studied. The result showed that the optimum liquid medium composition were sucrose 7.5 g·L-1, maltose 7.5 g·L-1, wheat bran 7.5 g·L-1, beef extract 3.5 g·L-1, KH2PO4 1.5 g·L-1, MgSO4·7H2O 0.5 g·L-1 and VB1 4 mg·L-1, natural pH. Through culturing for 96 h at 28℃ , the biomass of mycelium of Tremella sanguinea reached 4.29 g·L-1.

【中文關(guān)鍵詞】 血耳; 液體培養(yǎng)基; 菌絲體; 生物量; 正交設(shè)計(jì)
【英文關(guān)鍵詞】 Tremella sanguinea; Liquid medium; Mycelium; Biomass; Orthogonal design
【基金】天津市科學(xué)技術(shù)委員會(huì)項(xiàng)目(編號(hào):06ZHCXNC05900)。

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-018